Publications
Below, publications are listed
in reverse chronological order. After each I have started
writing a short description of the contents/significance of the paper.
2024
- Babu J N Reddy, Nathan Reddy Allipeta, Jun Allard, Steven P Gross
A new method to experimentally quantify dynamics of initial protein protein interactions;
Communications Biology 311 (7) PDF
2023
- Trini Nguyen*, Babu J N Reddy*, Steven P Gross, Christopher E Miles
ADP release can explain spatially-dependent kinesin binding times; bioRxiv https://doi.org/10.1101/2023.11.08.563482;PDF
*Co-primary authors
2022
- Ibtissem Nabti, Babu J N Reddy, Rachid Rezgui, Wenqi Wang, Steven P Gross, George T Shubeita
The ubiquitous microtubule-associated protein 4 (MAP4) controls organelle distribution by regulating the activity of
the kinesin motor; PNAS 119 (41), e2206677119 PDF
- Cody Combs, Daniel D Seith, Matthew J Bovyn, Steven P Gross, Xiaohui Xie, Zuzanna S Siwy;
Deep learning assisted mechanotyping of individual cells through repeated deformations and relaxations in undulating channels featured
Biomicrofluidics 16, 014104 (2022)PDF
2021
- M Bovyn, Babu J N Reddy, S Gross, J Allard
Diffusion of kinesin motors on cargo can enhance binding and run lengths during intracellular transport
Molecular Biology of the Cell 32 (9), 984-994PDF
- Leora Duong, Steven P. Gross* and Albert Siryaporn*
Developing Antimicrobial Synergy With AMPs;March 2021 | Volume 3 | Article 640981 PDF
2020
- Leora Duong, Steven P Gross, Albert SiryapornA novel antibacterial strategy: histone and antimicrobial peptide synergy
Microbial Cell 7 (11), 309PDF
- Tory Doolin, S P Gross, A Siryaporn
Physical mechanisms of bacterial killing by histones; Physical Microbiology, 117-133PDF
- Marta Bosch, Miguel Sánchez-Álvarez, Alba Fajardo, Ronan
Kapetanovic, Bernhard Steiner,
Filipe Dutra, Luciana Moreira, Juan Antonio López, Rocío
Campo, Montserrat Marí,
Frederic Morales-Paytuví, Olivia Tort, Albert Gubern, Rachel
M. Templin, James E. B. Curson,
Nick Martel, Cristina Català, Francisco Lozano, Francesc
Tebar, Carlos Enrich,
Jesús Vázquez, Miguel A. Del Pozo, Matthew J. Sweet,
Patricia T. Bozza,
Steven P. Gross, Robert G. Parton, Albert Pol,
Mammalian
lipid droplets are innate immune hubs integrating cell metabolism and host defense (Science
2020) PDF
- Tory Doolin, Henry M. Amir , Leora Duong, Rachel
Rosenzweig , Lauren A. Urban, Marta Bosch,
Albert Pol, Steven P. Gross; Albert Siryaporn,Mammalian histones facilitate
antimicrobial synergy by disrupting the bacterial proton gradient
and chromosome organization(Nature Communications
2020) PDF
2019
- Dail E. Chapman*, Babu J.N. Reddy*, Bunchhin Huy, Matthew
J. Bovyn, Stephen John S. Cruz,
Zahraa M. Al-Shammari, Han Han, Wenqi Wang, Deanna S. Smith
& Steven P. Gross
Regulation of in vivo dynein force production
by CDK5 and 14-3-3epsilon and KIAA0528 (Nature
Communications(2019) 10:228/
https://doi.org/10.1038/s41467-018-08110) [pdf],
*Co-primary authors
2018
- Byeongwook Lee, Dongkwan Shin,
Steven P. Gross, Kwang-Hyun Cho* Combined Positive and Negative Feedback Allows
Modulation of Neuronal Oscillation Frequency during
Sensory Processing (Cell Reports, 25, 1548–1560 (2018)) [pdf],
*Corresponding Author
- InSuk Joung, Jong Yun Kim, Steven P. Gross, Keehyoung Joo,
Jooyoung Lee*
Conformational Space Annealing explained: A general
optimization
algorithm, with diverse applications (Computer Physics
Communications 223 (2018) 28–33) [pdf],
*Corresponding Author
- Joseph M. Muretta†, Babu J. N. Reddy†, Guido Scarabelli†,
Alex F. Thompsone†, Shashank Jariwala,
Jennifer Major, Monica Venere, Jeremy N. Rich, Belinda
Willard, David D. Thomas, Jason Stumpff, Barry J. Grant,
Steven P. Gross and Steven S. Rosenfeld A posttranslational
modification of the mitotic kinesin
Eg5 that enhances its mechanochemical coupling and
alters its mitotic function (PNAS,
https://doi.org/10.1073/pnas.1718290115 ) [pdf],
† Equal Contribution
- Jared P. Bergman†, Matthew J. Bovyn†, Florence F. Doval,
Abhimanyu Sharma, Manasa V. Gudheti,
Steven P. Gross , Jun F. Allard#, and Michael D. Vershinin
#* ” Cargo navigation
across 3D microtubule intersections (PNAS,
www.pnas.org/cgi/doi/10.1073/pnas.1707936115) [pdf+Supp],
#,† Equal Contribution, *Corresponding authors.
2017
- Babu J N Reddy , Suvranta Tripathy, Michael Vershinin,
Marvin Tanenbaum, Jing Xu, Michelle
Mattson-Hoss, Karim Arabi, Dail Chapman, Tory Doolin,
Changbong Hyeon*, and Steven P. Gross* ” Heterogeneity
in Kinesin Function (Traffic, Volume 18, Issue 10 Pages
658–671 2017) [pdf+Supp],
*Corresponding authors.
- Yu CC, Reddy Babu J N, Wortman JC, Gross SP. ” Axonal Transport: A
Constrained
System J Neurol Neuromed (2017) 2(3): 20-24 [pdf]
- George Schubeita, Babu Reddy J N, Steven Gross (In Press)”
'Biophysics of Dynein in
vivo' Book Chapter:to appear in ’Dyneins:
Structure, Biology and Disease’
Volume 2 Dynein, Structure, Mechanics and Disease (King,
S.M.: editor) Elsevier inc.,(Nov 2017)
2016
- Babu J. N. Reddy & Steven Gross “Dynein: Let’s not
get stuck!” Cell Cycle,
DOI:10.1080/15384101.2016.1232085 (2016)[pdf]
- Babu
J.N. Reddy, Michelle Mattson, Caitlin L. Wynne, Omid Vadpey,
Abdo
Durra, Dail Chapman, Richard B. Vallee & Steven P.
Gross “Load-induced enhancement of Dynein force
production by
LIS1–NudE in vivo and in vitro”
Nature Communications Aug 4;7:12259 (2016) [pdf]
[Suppl]
2015
- Muretta
JM, Jun Y, Gross SP, Major J, Thomas DD, Rosenfeld SS. “The
structural
kinetics of switch-1 and the neck linker explain the
functions of
kinesin-1 and Eg5.”
PNAS (2015) 112(48):E6606-13[pdf]
- Mitra
Shojania Feizabadi, Babu Reddy J N, Omid Vadpey, Yonggun
Jun, Dail
Chapman, Steven Rosenfeld and Steven P. Gross“Microtubule
C-Terminal
Tails Can Change Characteristics of Motor Force Production”
Traffic(2015) [pdf]
- Albert
Herms, Marta Bosch, Babu J.N. Reddy, Nicole L. Schieber,
Alba Fajardo,
Celia Ruperez, Andrea Fernandez-Vidal, Charles Ferguson,
Carles
Rentero, Francesc Tebar, Carlos Enrich, Robert G. Parton,
Steven P.
Gross & Albert Pol.“AMPK activation promotes lipid
droplet
dispersion on detyrosinated microtubules to increase
mitochondrial
fatty acid oxidation”
Nature Communications. May 27,2015[pdf] [supp]
2014
- Suvranta
K Tripathy, Sarah J Weil, Chen Chen, Preetha Anand, Richard
B Vallee,
Steven P Gross“Autoregulatory mechanism for dynactin control
of
processive and diffusive dynein transport” Nature Cell Biology
1192–1201 (2014)[pdf]
- Yonggun
Jun, Suvranta K. Tripathy, Babu R. J. Narayanareddy,
Michelle K.
Mattson-Hoss and Steven P. Gross. “Calibration of Optical
Tweezers for
In Vivo Force Measurements: How do Different Approaches
Compare?” Biophys. J,
107:
1474-1484 (2014).[pdf]
- MK
Mattson-Hoss , Y Niitani , EA Gordon , Y Jun ,L Bardwell ,M
Tomishige ,
and SP Gross. “CK2 activates kinesin via induction of a
conformational
change.” PNAS,
111: 7000-5 (2014). [pdf]
- Babu R J
Narayanareddy, S Vartiainen ,N Hariri , DK O'Dowd , and SP
Gross. “A
Biophysical Analysis of Mitochondrial Movement: Differences
Between
Transport in Neuronal Cell Bodies Versus Processes.” Traffic, (2014). [pdf]
- A. Pol, S.P. Gross, and R.G. Parton. “The biogenesis of
the
multifunctional lipid droplet: lipids, proteins, and sites.”
J. Cell Biol.,
04(5):635-46 (2014). [pdf]
- J.
Wortman, U. Shrestha, D. Barry, M.L. Garcia , S.P. Gross+†,
and C.C.
Yu+†, “Axonal Transport: How High Microtubule Density Can
Compensate
for Boundary Effects in Small Caliber Axons”, Biophys. J.,
106(4):813-23 (2014). [pdf]
- A. Goulet, J. Major, Y. Jun, S.P. Gross, S.S. Rosenfeld,
C.A. Moores “A
comprehensive structural model of the mechanochemical cycle
of a
mitotic motor highlights molecular adaptations in the
kinesin family”, PNAS,111(5):1837-1842(2014).
[pdf]
[supp]
2013
- Kassan
A, Herms A, Fernández-Vidal A, Bosch M, Schieber NL, Reddy
Babu J N, Fajardo A, Gelabert-Baldrich M, Tebar F, Enrich C, Gross SP, Parton
RG, Pol A.,
“Acyl-CoA synthetase 3 promotes lipid droplet biogenesis in
ER
microdomains.”
J Cell Biol.
2013 Dec 23;203(6):985-1001. [pdf+Supp]
[pdf]
Determines new details into how LDs form in the
ER.
- A. Herms, M. Bosch, N. Ariotti, Babu J. N. Reddy, A.
Fajardo, A. Fernádez-Vidal, A. Alvarez-Guaita, M. A.
Fernádez-Rojo, C.
Rentero, F. Tebar, C. Enrich, M.-I. Geli, R. G. Parton, S.
P. Gross,
and A. Pol, “Cell to cell lipid droplet heterogeneity: a
population
advantage to reduce lipotoxicity” in
press Current
Biology(2013). [pdf]
[supp]
Discovers that cells actively create
heterogeneity in LDs, with some cells having few LDs and
some having
many. This decreases overall ROS, and the high-lipid cells
can supply
lipids to the low-lipid cells when needed.
- Juyong Lee, Steven P Gross and Jooyoung Lee, “Improved
network community structure improves function prediction” in press Scientific Reports(2013).
Uses an improved minimization approach to better
implement maximization of Q, resulting in improved
community detection.
This is applied to protein-interaction networks, and the
resulting
detected communities are then used as additional
information in
predicting protein function.
- K.P. Bohannon, Y. Jun, S.P. Gross, and G.A. Smith,
“Differential protein partitioning within the herpesvirus
tegument and
envelope underlie a complex and variable virion
architecture.”, PNAS,110(17):E1613-20(2013).
[pdf]
[supp]
Uses a combination of high-resolution image
analysis and genetic engineering to analyze the structure
and
organization of herpes viruses.
- R.P. Erickson, S.P. Gross+†, and C.C.P. Yu+†
“Filament-filament switching can be regulated by separation
between
filaments together with cargo motor number.”, PLoS One.
8(2):e54298 (2013). [Link]
Investigates theoretically what happens when a
3D
cargo with multiple motors arrives at a filament-filament
crossing. It
discovers that the outcome is sensitive to filament
spacing.
- S. P. Gross “Come together: group behavior of Dynein
motors.”, Dev Cell.
Jan 28;24(2):117-8. (2013)
A news and views discussing a nice recent paper
by Roop Mallik’s group, suggesting how specific
single-molecule
adaptations might contribute to ensemble function for
multiple dynein
motors.
2012
- P. Anand, S. Cermelli, Z. Li, A. Kassan, M. Bosch, R.
Sigua, L. Huang, A.J. Ouellette, A. Pol, M.A. Welte and S.P.
Gross, "A
novel role for lipid droplets in the?organismal
antibacterial
response", eLife, Nov. 13, (2012). [pdf]
Discovers what we believe to be a new pathway in
innate immunity, where anti-bacterial histone proteins are
sequestered
on cytoplasmic lipid droplets, and then released in the
presence of
cytosolic bacteria, to kill the invaders. Please also see
the
accompanying "insight" article by Roberto Kolter:[pdf]
- J. Xu, Z. Shu, S.J. King, and S.P. Gross, "Tuning
Multiple Motor Travel Via Single Motor Velocity", Traffic,
March
27, (2012). [pdf]
Shows that for multiple-motor based transport,
velocity can be used to control mean travel. For single
kinesin motors,
processivity is independent of velocity, which means that
the slower
you go, the lower the single-motor off rate. Thus, for
multiple motors
acting together, since the "on" rate is independent of
velocity, but
the off-rate is not,?the ratio of on-rate to off-rate can
be tuned by
velocity, and this can be used to quite dramatically alter
how far the
group of motors takes the cargo.
- J.Lee, S. P. Gross, and J. Lee, "Modularity
optimization by conformational space annealing", Phys.
Rev.
E, 85, (2012).
Applies a new approach to detect hidden
communities within graphs, resulting in improved
modularity and more
rapid detection of the communities
- R.Sigua, S. Tripathy, P. Anand+†,and S.P.
Gross+†, "Isolation and Purification of Kinesin from
Drosophila
Embryos", J. Vis. Exp. (62), e3501, DOI:
10.3791/3501 (2012). [Link
to
JOVE Site]
Provides a purification protocol to help
biophysics-types purify functional kinesin from fly
embryos for
single-molecule experiments
- J.Xu, Babu J N Reddy*, P. Anand,*, Z. Shu,*, S.
Cermelli,M.K. Mattson, S.K. Tripathy, M.T. Hoss, N.S. James,
S.J. King,
L. Huang, L. Bardwell and S.P. Gross+", "Casein kinase 2
reverses
tail-independent inactivation of kinesin-1", Nature
Communications, March 27, (2012). [pdf] [supp]
Discovers a new pathway of kinesin
inactivation/reactivation. Shows that kinesin by itself
tends to go
inactive, and that this inactivation can be reversed by
the presence of
CK2, both in vitro and in vivo. Importantly, this new
inactivation/reactivation pathway does NOT involve
autoinhibition of
kinesin via the established head-tail inactivation
pathway, as even
tail-less kinesin goes inactive.
- G. T. Shubeita and S. P. Gross, "Intracellular Transport:
Relating Single-Molecule Properties to in Vivo Function" in
E. Egelman (Ed.) Comprehensive
Biophysics, Elsevier, (2012).
Review
2011
- A. Kunwar, Kunwar, S.K. Tripathy, J. Xu, M.K. Mattson, P.
Anand, R. Sigua, M. Vershinin, R.J. McKenney, C. C. Yu, A.
Mogilner+†,
and S.P. Gross+†, "Mechanical stochastic tug-of-war models
cannot
explain bidirectional lipid-droplet transport", PNAS,
November
14, (2011). [pdf]
Investigates theoretically how multiple kinesin
and dynein motors function together. By comparing theory
and
experiments, it concludes that straightforward mechanistic
tug-of-war
scenarios are inconsistent with observed motion for
bi-directional
lipid droplet motion, suggesting additional coordination
mechanism
likely exist.
- J. Yi, K. Ori-McKenney, R. McKenney, M. Vershinin, S.P.
Gross, and R. B. Vallee, "High Resolution Imaging
Reveals
Indirect Coordination of Opposite Motors and LIS1 Role in
High-load
Axonal Transport.", J. Cell Biol.,
(2011).?[pdf]
A paper following-up on the in-vitro study (#53,
below) of Dynein"s regulation by NudE and Lis1, but this
time looking
at transport in cells. The main points are 2. First, that
coordination
between opposite motors (for bi-directionally moving
transport) is NOT
directly and immediately mediated by activity of the
motors. That is,
when dynein (minus-end) activity is decreased rapidly (by
acute
inhibition), plus-end motion is inhibited, but with a
temporal delay.
Thus, however motor inactivation is sensed (potentially by
force
production, or other mechanisms), once detected,
inactivation of the
opposite motors requires some additional feedback
mechanism. This is
consistent with the fact that in vitro, neither kinesin or
dynein
require opposite-polarity motors to be present to
function. The second
point of the paper is that in neurons, larger cargos
appear likely to
be exposed to significantly more opposition to motion, so
that their
appropriate transport requires NudE/Lis1 function, but
this?requirement
is much less for smaller cargos.
- M. Bosch, M. Mar? S.P. Gross, J.C. Fern?dez-Checa, and A.
Pol, "Mitochondrial cholesterol: a connection between
caveolin and
metabolism", Traffic (AOP)?(2011). [pdf] ?
?A short review/opinion piece with a bit of new
data, furthering the idea presented in #56 below, i.e.
that that loss
of CAV1 function can result in multiple disease phenotypes
by affecting
Mitochondrial function, in particular by altering
cholesterol in the
mitochondrial membrane.
- R.P. Erickson, Z. Jia, S.P. Gross+† and C.C. Yu+†, "How
Molecular Motors are Arranged on a Cargo is Important for
Vesicular
Transport", PLOS Computational Biology
(2011).?strong> +=Co-senior author
†=Corresponding author [pdf]
?
A theoretical study
investigatingthree-dimensional aspects of cargo motion.
- J. Xu and S.P. Gross, "Biophysics of dynein in vivo"
(invited review), to appear in Dyneins: Structure,
Biology and Disease, (Elsevier), Editor Stephen
M. King
(2011).
Review
- M. Bosch, M. Mar? A. Herms, A. Fern?dez, A. Fajardo, A.
Kassan, A. Giralt, A. Colell, D. Balgoma, E. Barbero, E.
Gonz?ez-Moreno, N. Matias, F. Tebar, J. Balsinde, M. Camps,
C. Enrich,
S.P. Gross, C. Garc?-Ruiz, E. P?ez-Navarro, J.C.
Fern?dez-Checa, and A.
Pol, "Caveolin-1 deficiency causes cholesterol dependent
mitochondrial
dysfunction and apoptotic susceptibility" , Current
Biology (AOP)?2011). [pdf] [supp]
Discovers that loss of CAV1 function can result
in multiple disease phenotypes by affecting Mitochondrial
function.
CAV1 is discovered to contribute to mitochondrial
cholesterol
homeostasis, and by altering cholesterol in the
mitochondrial
membrane,?loss of CAV1 function thus results in a variety
of
alterations of mitochondrial function.
2010
- K.M. Ori-McKenney*, J. Xu*, S.P. Gross+†, and R.B.
Vallee+†, "A cytoplasmic dynein tail mutation impairs motor
processivity", Nature Cell Biology (AOP),
(2010) [pdf]
[supp]
Determines the mechanistic effects of the LOA
mutation, a mutation in the dynein heavy chain, that leads
to an
ALS-like phenotype in heterozygous mice bearing the
mutation.
Homozygous mice die at birth. The paper has a number of
main findings.
First, that the mutation decreases single-molecule
processivity.
Second, that this defect carries over to multiple-motor
transport, and
the effects on processivity at the single-molecule can
quantitatively
explain?the observed impairment in multiple-motor based
transport,
likely resulting in the observed disease phenotype. This
study thus
provided the first direct experimental evidence supporting
the
long=hypothesized importance of single-motor processivity
for healthy
transport. The study also suggests a role for the tail in
controlling
enzymatic coordination between the two heads. Because this
loss of
enzymatic coordination correlates with increased
side-to-side stepping
of the motor, and hence less lateral contact between the
two heads, we
believe this is consistent with a mechanism whereby dynein
heads
communicate with each other laterally.
?strong> *=Co-primary author, +=Co-senior
author †=Corresponding author
- R.J. McKenney*, M. Vershinin*, A. Kunwar, R.B. Vallee+†,
and S.P. Gross+†, "LIS1 and NudE Induce a Persistent Dynein
Force-Producing State", Cell 141: 304"314,
(2010) [pdf]
[supp]
Discovers the mechanistic role of NudE and Lis1.
Briefly, NudE alone both recruits dynein to a particular
location, but
also inactivates it. Lis1 then binds NudE, and the
combined
Dynein-NudE-Lis1 complex is again active, but now with
improved
performance. In particular, the complexes processivity is
increased,
and more importantly, detachment under load is decreased.
This allows
the motors to "hold on" under load, and is critical in
allowing
multiple motors to work well together. In the presence of
NudE and
Lis1, the same number of motors can exert a higher average
force, not
because each motor exerts more force, but rather because
on average
more motors are engaged (they don"t fall off).
*=Co-primary author, +=Co-senior
author †=Corresponding author
2009
- K.H. Bremner, J. Scherer, J. Yi, M. Vershinin, S.P.
Gross,
and R.B. Vallee, "Adenovirus transport via direct
interaction of
cytoplasmic dynein with the viral capsid hexon subunit". Cell
Host
Microbe. 6(6):523-35. (2009) [pdf]
Investigates how dynein moves Adenovirus
particles.
- M. Ingelmo-Torres, E. Gonz?ez-Moreno, A. Kassan, M.
Hanzal-Bayer, F. Tebar, A. Herms, T. Grewal, J.F. Hancock,
C. Enrich,
M. Bosch, S.P. Gross, R.G. Parton and A. Pol, "Hydrophobic
and Basic
Domains Target Proteins to Lipid Droplets", Traffic
10(12):1785-801 (2009) [pdf]
Investigates how proteins such as Caveolin are
targeted to Lipid Droplets.
- R. Mallik and SP Gross, "Intracellular transport: how do
motors work together"", Curr Biol.19(10), (2009) [pdf] ?
A short dispatch on interesting work
investigating transport of intra-flagellar particles.
- F. Ziebert, M. Vershinin, SP Gross, IS Aranson,
"Collective alignment of polar filaments by molecular
motors." Eur
Phys J E Soft Matter, 28(4), (2009) [pdf]
Investigates theoretically and experimentally
how
molecular motors can align filaments by cross-linking and
moving.
- K. Larsen, J. Xu, S. Cermelli, and S.P. Gross, "BicaudalD
actively regulates microtubule motor activity in lipid
droplet
transport.", PLoS one 3(11), (2009). [pdf] ?
Establishes that BicD plays a role in regulating
lipid droplet motion in drosophila embryos, and shows that
BicD plays a
dynamic rather than static role in controlling such
motion.
2008
- G. Shubeita, S. Tran, J. Xu, M. Vershinin, S. Cermelli,
S.
Cotton, M. Welte, and S.P. Gross, "Consequences of motor
copy number on
the intracellular transport of kinesin-1-driven lipid
droplets"
Cell ?35(6), (2008). [pdf] ?[supp]
Establishes that for lipid droplets, simply
controlling the overall number of motors does not result
in changes to
droplet motion.
- A. Kunwar, M. Vershinin, J. Xu, and S.P. Gross,
"Stepping,
Strain Gating, and an Unexpected Force-Velocity Curve for
Multiple-Motor-Based Transport", Current Biology 18,
1"11, August 26, 2008 (2008). [pdf] ?[supp]
Investigates theoretically how multiple kinesin
motors function together, and how uneven load sharing can
result in
enhances system performance under load. It finds that
ensemble multiple
motor function depends strongly on the coupling between
the motors. It
predicts"which is then confirmed experimentally"that
surprisingly, for
a range of likely cytosolic viscosities, cargos driven by
a single
motor can move faster than cargos moved by two or more
motors.
- M.A. Welte and S. P. Gross,?"Molecular motors: a traffic
cop within"", HFSP Journal in press,
(2008). [pdf]
A brief review discussing a paper from the
Lipowsky group that recently appeared in PNAS. The work is
very
intriguing, in that it presents a model that suggests that
many aspects
of bi-directional vesicular motion may be explained
quantitatively due
to specific properties of single motors, and how
tug-of-wars are
resolved.?/blockquote>
- M. Vershinin, J. Xu, D. Razafsky, S.J. King, and S.P.
Gross, "Tuning microtubule-based transport through
filamentous MAPs:
the problem of dynein." Traffic, 9(6):882-92
(2008).
[pdf]
A previous PNAS paper (#38, below) investigated
how two or three kinesin motors function together, and
showed that
tau"at levels found in cells"can function to regulate the
number of
engaged motors, allowing cells the possibility to
spatially regulate
plus-end transport via control of track (microtubule)
accessibility.
This raised the potential problem of cross-talk between
plus-end and
minus-end transport, since they both occur along
microtubules. In this
manuscript we investigate dynein"s sensitivity to tau, in
both the
single- and multiple-motor regimes. We show that
filament-level
regulation can occur without cross-talk, because dynein is
essentially
unaffected by the low levels to moderate levels to tau
that so
significantly alter kinesin-based transport. Using a
construct (a
portions of dynein"s microtubule-binding domain), we
investigate how
dynein avoids kinesins" tau sensitivity.
- B.C. Carter, M. Vershinin, S.P. Gross, "A Comparison of
Step-Detection Methods: How Well Can You Do"" Biophys.
Jl,94(1):306-19, (2008). [pdf]
Investigates properties of different
step-detection methods, and then applies the best one to
the problem of
how multiple kinesin motors function together. It shows
that under low
load, and saturating ATP, in vitro two kinesin motors
attached to a
cargo do not coordinate, but instead function
independently, so that
the center of mass of the cargo moves in ~4nm steps.
2007
- S. P. Gross, M. Vershinin and G.T. Shubeita,?"Cargo
Transport: Two Motors Are Sometimes Better Than One", Curr.
Bio.
v.17, R478-486 (2007). [pdf]
A review of advances in our understanding of how
multiple motors move cargos, and the ramifications of the
number of
engaged motors moving cargos. Based on a summary of
structural (EM
data) and in vivo force measurements, it suggests that
most cargos
transported along microtubules are moved by a limited
number of motors
(between 1 and 5).
- S. P. Gross,?"Molecular Motors: A Tale of Two Filaments",
Curr.
Bio. v.17, R277-280 (2007). [pdf]
A brief review of advances in myosin V-actin
filament-filament switching, discussing both the role of
the number of
motors on the cargo, and also new results on the
properties of single
Myosin-V motors.
- D.Y. Petrov, R. Mallik, G.T. Shubeita, M. Vershinin, S.P.
Gross †+, and C.C.Yu+ ,"Studying Molecular Motor-based Cargo
Transport:
What is Real, and What is Noise"", Online Early Edition,?Biophys.
Jl, (2007).
[pdf]? [supp]?+=Co-senior
author
†=Corresponding author
Investigates how two or three kinesin motors
function together, and shows that stall forces for motors
are additive,
and that multiple kinesin motors move cargos very long
distances. It
then shows that tau"at levels found in cells"can function
to regulate
the number of engaged motors, allowing cells the
possibility to
spatially regulate transport via control of track
(microtubule)
accessibility.
- J.E. Martinez, M.D. Vershinin , G.T. Shubeita, and S.P.
Gross, "On the use of in vivo cargo velocity as a
biophysical marker", Biochem.
Biophys. Res. Comm. 353, 835-840 (2007). [pdf]? [Word, suppl]
[jpeg]
Investigates, both theoretically and
experimentally, the published proposal that a cargo"s
velocity can be
used to infer the number of engaged motors moving the
cargo. The
manuscript concludes that cargo velocity is likely a poor
marker for
the number of engaged motors.
- M. Vershinin, B.C. Carter, D.S. Razafsky, S.J. King and
S.P. Gross, "Multiple-motor based transport and its
regulation by Tau",
PNAS V. 104, 87"92 (2007). (track 2) [pdf] ?[supp]
Investigates how two or three kinesin motors
function together, and shows that stall forces for motors
are additive,
and that multiple kinesin motors move cargos very long
distances. It
then shows that tau"at levels found in cells"can function
to regulate
the number of engaged motors, allowing cells the
possibility to
spatially regulate transport via control of track
(microtubule)
accessibility.
2006
- R. Mallik and S. P. Gross,?"Molecular motors as cargo
transporters in the cell "The good, the bad and the
ugly",?Physica A,
V. 372,?65 "69, (2006). [pdf]
A brief review of the function of molecular
motors.
- S. Cermelli*, Y. Guo,?S.P. Gross+† and?M.A. Welte+, "The
Lipid-Droplet Proteome Reveals that Droplets Are a
Protein-Storage
Depot", Curr. Bio., v. 16, 1783-1795, (2006). [pdf] [supp] +=Co-senior
author?/span>?†=Corresponding author
Uses mass spectrometry to determine the
proteins
present on embryonic lipid droplets.?Because the
proteins present are
found to be highly conserved between Drosophila and
mammalian droplets,
this suggests that studying the Drosophila droplets can
provide
important insights into the similar processes in
mammals. In the
proteome, certain unexpected proteins"histones"are
present in large
amounts The localization of histones to the droplets is
investigated in
depth, and concluded to be real, and temporally
regulated. Based on
these findings, together with published observations
from others, the
manuscript proposes a new model for lipid droplets as
generalized sites
of protein storage/sequestration.
- S. L. Bullock, A. Nicol, S.P. Gross, and D. Zicha, "
Guidance of Bidirectional Motor Complexes by mRNA Cargoes
through
Control of Dynein Number and Activity", Curr. Bio.,V. 16,
1447"1452,
(2006). [pdf]
- S.E. Antinone, G.T. Shubeita, K.E. Coller, J.I. Lee, S.
Haverlock-Moyns, S.P. Gross+, and G.A. Smith+, "The
herpesvirus capsid
surface protein, VP26, and the majority of the tegument
proteins are
dispensable for capsid transport toward the nucleus", J.
Virol., n. 80,
5494"5498 (2006). [pdf]
2005
- Roop Mallik, Dmitri Petrov, S.A. Lex, S.J. King, and S.P.
Gross, "Building Complexity: An In Vitro Study of
Cytoplasmic Dynein
with In Vivo Implications", Curr. Bio., v. 15, 2075-2085,
(2005). [pdf] [supp]
Investigates how two or three dynein motors
function together, and shows that stall forces for motors
are additive,
and that multiple dynein motors move cargos very long
distances. Shows
that a cargo moved by two dyneins is expected to move a
very long
distance, so that for cargos moved by two or more dynein
motors in
cells, the dynactin complex (which increases dynein
processivity) is
likely unnecessary as far as facilitating travel distance.
This does
not?mean, however, that the dynactin complex is
unimportant"we have
previously shown that in some cases it plays a role in
coordinating
kinesin and dynein, and others have shown that it
frequently plays an
important role in dynein-cargo attachment.
- M.P. Singh, R. Mallik, S. P. Gross ?, and C.C. Yu ?,
"Monte Carlo modeling of single molecule cytoplasmic
dynein", PNAS, v.
102, 12059"12064, (2005). (?=co-senior author) [pdf]
- M.A. Welte*, S. Cermelli*, J. Griner, A. Viera, Y. Guo,
D.
Kim, J.G. Gindhart , S.P. Gross, "Regulation of
lipid-droplet transport
by the Perilipin homologue LSD2", Curr. Bio., v. 15,
1266-1275, (2005). [pdf] [supp]
- F. Lin, CM Nguyen, SJ Wang, W Saadi, SP Gross, NL Jeon,
"Neutrophil Migration in Opposing Chemoattractant Gradients
Using
Microfluidic Chemotaxis Devices", Ann. Biom. Engin., v. 33,
no. 4,
475-482 (2005). [pdf]
- Brian C. Carter, George T. Shubeita, and Steven P. Gross,
" Tracking single-particles: a user-friendly quantitative
evaluation",
Physical Biology 2, 60"72, (2005). [pdf]
- T. del Rio, T.H. Ch"ng 2, E.A. Flood, S.P. Gross, and
L.W.
Enquist " Heterogeneity of a fluorescent tegument component
in single
psedorabies virons and enveloped axonal assemblies", J.
Virol. , n. 79,
3903-19 (2005). [pdf]
2004
- Roop Mallik and Steven P. Gross, "Molecular Motors:
Strategies to Get Along", Current Biology, v. 14, R971-R982,
(2004). [pdf]
- G.A. Smith, L. Pomeranz, S.P. Gross?and L. Enquist?
"Local
modulation of plus-end transport targets herpesvirus entry
and egress
in sensory axons", PNAS early eddition, (2004) (?=co-senior
author) [pdf]
- J. Snider, F. Lin, N. Zahedi, V. Rodionov, C.C. Yu? and
S.P. Gross? "Intracellular actin-based transport: How far
you go
depends on how often you switch", PNAS 101: 13204"13209,
(2004) (?
=co-senior author) [pdf]
[supp]
- SP Gross "Hither and yon: a review of bi-directional
microtubule-based transport", Physical Biology 1: R1"R11,
(2004) [pdf]
- F. Lin, CM Nguyen, SJ Wang, W Saadi, SP Gross? NL Jeon?
"Effective neutrophil chemotaxis is strongly influenced by
mean IL-8
concentration", Biochem. Biophys. Res. Commun. Jun
25;319(2):576-81
(2004). (?=co-senior author) [pdf]
- R. Mallik, B.C. Carter, S.A. Lex, S.J. King and S.P.
Gross
"Cytoplasmic dynein functions as a gear in response to
load", Nature
427, 649-52 (2004). [pdf]
2003
- Vladimir Rodionov, Julie Yi, Anna Kashina, Abiola
Oladipo,
and Steven P. Gross, "Switching between microtubule- and
actin-based
transport systems in melanophores is controlled by cAMP
levels",
Current Biology, v. 13, 1837"1847, (2003). [pdf]
- Steven P. Gross,Yi Guo, Joel E. Martinez, and Michael A.
Welte, "A Determinant for Directionality of Organelle
Transport in
Drosophila Embryos", Current Biology, v. 13, 1660"1668,
(2003). [pdf]
- S. P. Gross, "Dynactin: Coordinating Motors with Opposite
Inclinations (Dispatch)", Current Biology, v. 13, R320-322
(2003).
[pdf]
- S. P. Gross, "Application of Optical Traps In Vivo",
Methods in Enzymology, v. 361, 162-174 (2003). [pdf]
2002
- L.J. Davis, D.J. Odde, S. M. Block, and S. P. Gross, "The
Importance of Lattice Defects in Katanin-Mediated
Microtubule Severing
in Vitro", Biophys. J. 82, 2916-27 (2002). [pdf]
- S. P. Gross*, M. C. Tuma*, S. W. Deacon, A. S.
Serpinskaya
A. R. Reilein and V. I. Gelfand, "Interactions and
Regulation of
Molecular Motors in Xenopus Melanophores", J. Cell Bio. 156,
855-65
(2002). [pdf]
- S.P Gross*, M. Welte*, S.M. Block, and E.F. Wieschaus,
"Coordination of opposite-polarity microtubule motors", J.
Cell Bio.
156, 715-24, (2002) [pdf]
- L.W. Enquist, M.J. Tomishima, S. Gross, G.S. Smith,
"Directional spread of an alpha-herpsesvirus in the nervous
system",
Veter. Microb. 2266, 1-12 (2002). [pdf]
2001
- G.A. Smith*, S.P Gross*, and L.W. Enquist, "Herpesviruses
use bidirectional fast-axonal transport to spread in sensory
neurons",
PNAS 98 3466-70 (2001) [pdf]
1987~2000
- S.P Gross, M. Welte, S.M. Block, and E.F. Wieschaus,
"Dynein-mediated cargo transport In vivo. A switch controls
travel
distance.", J. Cell Biol. 148 945-56 (2000). [pdf]
- S.P. Gross*, M. Welte*, M. Postner, S. M. Block, and E.F.
Wieschaus, "Developmental and Genetic Regulation of Vesicle
Transport
in Drosphila Embryos", Cell 92, 547 (1998). [pdf]
- K. Visscher, S. P. Gross, and S. M. Block, "Construction
of Multiple-Beam Optical Traps with Nanometer-Resolution
Position
Sensing", IEEE Jl. Sel. Top. Quant. Electr., 2, 1066 (1996).
[pdf]
- E. Sharon, S. P. Gross, and J. Fineberg, "Energy
Dissipation in Dynamic Fracture," Phys. Rev. Lett., 76, 2117
(1996).
- S.P. Gross, "Instabilities in Fast Fracture", Ph.D.
Dissertation, University of Texas, (1995)
- E. Sharon*, S. P. Gross*, and J. Fineberg, ``Local Crack
Branching as a Mechanism for Instability in Dynamic
Fracture,'' Phys.
Rev. Lett, 74, 5096 (1995).
- M. Marder and Steve Gross, ``Origin of Crack Tip
Instabilities,'' Jl. of the Mech. and Phys. of Sol., 43, 1
(1995).
- S. P. Gross, J. Fineberg, M. Marder, W.D. McCormick, and
H. L. Swinney, ``Acoustic Emissions from Rapidly Moving
Cracks," Phys.
Rev. Lett., 71, 3162 (1993).
- J. Fineberg, S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in the propagation of fast cracks'', Phys.
Rev. B, 45,
5146, 1992.
- J. Fineberg, S. P. Gross, M. Marder, and H. L. Swinney,
``Instability in Dynamic Fracture,'' Phys. Rev. Lett., 67,
457, (1991).
- S. Gross, G. Zocchi, and A. Libchaber ``Waves and Plumes
of thermal boundary layer,'' C. R. Acad. Sci. Paris, 307,
Serie II,
447, (1988).
- J. Glazier, S.P. Gross, J. Stavans, ``Dynamics of
two-dimensional soap froths'', Phys. Rev. A, 36, 306, 1987.
*= Joint first authors.